3-Morpholinopropanesulfonic acid, abbreviated as MOPS, is an amphoteric buffer reagent commonly used in biochemistry and molecular biology. MOPS itself belongs to Good’s buffers and can be used not only as a buffer system for cell culture media, but also as a common buffer for electrophoresis and protein purification chromatography.

What experiments can MOPS be used for?

1. as a running buffer in electrophoresis and for protein purification in chromatography.

2. formulated into a variety of agar media for the culture of bacteria, yeast and mammalian cells. However, concentrations above 20 mM are not recommended for mammalian cells.

3. used as a lysis buffer for E. coli cells.

4. as an eluent in gel filtration chromatography.

5. applied to Northern hybridization, as a buffer for the separation of RNA and during membrane transfer.

6. applied to the determination of diquinolinecarboxylic acid (BCA).

7. for electron transfer and phosphorylation studies of chloroplast thin-layer preparations.

8. It can be used in biochemical diagnostic kits, such as DNA/RNA extraction kits, PCR kits, kits for the determination of creatinine, etc.

Biological buffer in barrel

MOPS transportation and storage

MOPS powder can be stored at room temperature for several years. MOPS solutions can be stored at 2-8°C for at least 6 months, and are sterilized by filtration through a 0.2 μm membrane; autoclaving is not recommended. For preparation of MOPS solution without nuclease, the water can be autoclaved before adding the powder to dissolve.

Characteristics of MOPS buffer

(1) Good solubility in aqueous solution, colorless and transparent in aqueous solution.

(2) chemically stable, no toxicity to organisms and no obvious permeation effect on biological membranes.

(3) stable acid-base dissociation constant (pKa value), generally in the range of 6-8, stable ability to release protons, and less affected by ion concentration, solution composition and temperature.

(4) Not a metabolite, activator or inhibitor of biochemical reaction series, nor a substrate for enzymes, i.e., does not react with a variety of enzymes and chemical reagents, and the cells exhibit high viability when used in tissue culture, virus diagnosis and other tests.

(5) In the ultraviolet wave actually no characteristic absorption, in the wavelength of less than 230-240nm only weak absorption, and therefore does not interfere with the usual photometric measurements.

(6) No complexing ability or weak complexing ability to cations.

MOPS powder

Amphoteric ionic buffers have many advantages, wide application and market demand, and have been widely produced. Dexing Technology can provide a variety of high-quality amphoteric ionic buffers, including HEPES, MOPS, CAPS and other advantageous products